Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants

نویسندگان

  • A. Kumar Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
  • A. V. Singh National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra-282 001, India
  • B. Singh Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
  • K. K. Chaubey Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
  • N. Kumar Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
  • P. K. Singh National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra-282 001, India
  • S. Gupta Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
  • S. V. Singh Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
چکیده مقاله:

Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimationof prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock withclinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was substantial.1262 faecal samples from animals suspected of clinical JD were screened by microscopy in 2009-2010 and41.3% were positive. Prevalence of MAP was highest in cattle (61.8%), followed by goats (41.3%), sheep(33.4%) and buffaloes (15.7%). Faecal microscopy was cost effective, easy to adopt and repeatable for thescreening of domestic ruminant population against MAP infection.

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منابع مشابه

comparison of microscopy and blood-pcr for the diagnosis of clinical johne’s disease in domestic ruminants

microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

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عنوان ژورنال

دوره 14  شماره 4

صفحات  345- 349

تاریخ انتشار 2013-12-30

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